C9249

CryoSOfree^™ DMSO-free Cryopreservation Medium

• Synonym(s): cell freezing medium
• NACRES: NA.75
• UNSPSC Code: 12352207

Properties

• form: liquid
• Quality Segment: 200
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): cell culture | mammalian: suitable, cryopreservation: suitable
• greener alternative category: , Aligned
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree^™ DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree^™ DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.

Application

CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown[1][2] to preserve the potential of stem cell for differentiation.

Features and Benefits

• Preserves pluripotency of stem cells
• Low cytotoxicity
• Chemically defined, serum free, protein free
• Ready to use

The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63

Preparation Note

1 ml of CyroSOfree is sufficient for 5x10⁵- 5x10⁶ cells.

Legal Information

CryoSOfree is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290,H315,H319
• pcodes: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 10 - Combustible liquids