A9228

Amyloglucosidase from Rhizopus sp.

≥40,000 units/g solid

• Synonym(s): 1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase
• CAS Number: 9032-08-0
• Enzyme Commission number: 3.2.1.3 (BRENDA, IUBMB)
• EC Number: 232-877-2
• MDL number: MFCD00081350
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized solid
• Quality Level: 200
• specific activity: ≥40,000 units/g solid
• solubility: 0.03 M sodium citrate-phosphate buffer, pH 4.5: soluble 5.0 mg/mL
• storage temp.: −20°C

Description

Application

Amyloglucosidase is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Rhizopus sp., has been used to study the cleavage of oligosaccharides during ER-associated degradation of proteins (ERAD). The enzyme has been used in the glycosylation of N-vanillyl-nonanamide to form a water-soluble component with pharmacological applications. The glycogen was estimated in stipe residue of Coprinus cinereus by treating with amyloglucosidase and measuring the amount of glucose produced.

Biochem/physiol Actions

Amyloglucosidases are capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Unit Definition

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Physical form

Lyophilized salt free powder

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves