A3813

Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Goat Anti-Human Kappa Light Chains (Bound and Free)−AP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162518
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: DB, ELISA (d), IHC (p)
• Citations: 24

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: human
• technique(s): direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... IGK@(50802)

Description

General description

Human κ light chains are antibody fragments that regulate immunological responses in cells. Increased expression of κ light chains has been linked to chronic lymphocytic leukemia . The variable fragment of the κ light chain gene has also been associated with vaccine-induced antibody response to the polysaccharide coat in Haemophilus influenzae type b . Anti-Human κ Light Chains (Bound and Free-Alkaline Phosphatase antibody is specific for human κ light chain when tested against human IgA, IgG, IgM, IgGλ, IgAλ, IgMλ, Bence Jones κ and λ myeloma proteins.

Immunogen

Purified Bence Jones κ light chain

Application

Anti-Human κ Light Chains (Bound and Free)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for dot blot (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Mouse-Human chimeric IgG′s specific for Pseudomonas aeruginosa serogroup 06 lipopolysaccharide were identified by ELISA in transfected CHO cell supernatants using Alkaline phosphatase conjugated goat anti-Human Kappa Light chains.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable