94281
Urease from Canavalia ensiformis (Jack bean)
powder, white, ~8 U/mg
Synonym(s):
Jack bean urease, Urea amidohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Specific activity:
~8 U/mg
Biological source:
Canavalia ensiformis
Pricing and availability is not currently available.
biological source
Canavalia ensiformis
Quality Segment
form
powder
specific activity
~8 U/mg
mol wt
Mr ~480000
color
white
storage temp.
−20°C
General description
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants.
Application
Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes [1]. It may be useful for the detection of pathogens as well as heavy-metal ions.
Biochem/physiol Actions
Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit [2].
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C
1 of 1
This Item | |||
|---|---|---|---|
| biological source Canavalia ensiformis | biological source Canavalia ensiformis | biological source - | biological source Canavalia ensiformis |
| specific activity ~8 U/mg | specific activity 15,000-50,000 units/g solid | specific activity 50,000-100,000 units/g solid | specific activity - |
| form powder | form powder | form powder | form solid |
| mol wt Mr ~480000 | mol wt ~544620 Da | mol wt ~544620 Da | mol wt hexamer 545 kDa, trimer ~272 kDa |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| color white | color - | color - | color - |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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