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79189

Sigma-Aldrich

Abberior® FLIP 565, NHS ester

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

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About This Item

UNSPSC Code:
12352200

Quality Level

form

solid

concentration

≥60.0% (degree of coupling)

solubility

DMF: 1 mg/mL, clear

fluorescence

λem 580 nm±5 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum (off-state) λmax:314 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)

Application

Abberior® FLIP 565 conjugated with secondary antibody has been used for STORM (stochastic optical reconstruction microscopy) imaging of COS-7 and S180 cells.

Suitability

Designed and tested for fluorescent super-resolution microscopy

Legal Information

abberior is a registered trademark of Abberior GmbH

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Remi Galland et al.
Nature methods, 12(7), 641-644 (2015-05-12)
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Stefan W Hell
Nature biotechnology, 21(11), 1347-1355 (2003-11-05)
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to

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