07985
6-Aminofluorescein
BioReagent, suitable for fluorescence, ~95% (HPLC)
Synonym(s):
Fluoresceinamine isomer II
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About This Item
Empirical Formula (Hill Notation):
C20H13NO5
CAS Number:
Molecular Weight:
347.32
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
257-334-7
MDL number:
Beilstein/REAXYS Number:
51708
product line
BioReagent
Quality Level
assay
~95% (HPLC)
form
powder
impurities
≤5% 5-aminofluorescein
mp
285 °C (dec.) (lit.)
solubility
DMSO: soluble, acetone: soluble, methanol: soluble
fluorescence
λex 490 nm; λem 520 nm in 0.1 M Tris pH 9.0
suitability
suitable for fluorescence
SMILES string
Nc1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1
InChI
1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2
InChI key
YOAWSYSKQHLFPM-UHFFFAOYSA-N
Application
6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed ‘buckysomes′. 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein.
Other Notes
Note: Do not confuse with fluorescamine.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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K C Ingham et al.
Biochimica et biophysica acta, 670(2), 181-189 (1981-09-29)
A method for covalent attachment of a fluorescent molecule to the carbohydrate moieties of glycoproteins is described. The glycoproteins were oxidized with periodate under mild conditions selective for sialic acid (Van Lenten, L. and Ashwell, G. (1971) J. Biol. Chem.
I Climent et al.
Analytical biochemistry, 182(2), 226-232 (1989-11-01)
Oxidative modification of proteins is implicated in a number of physiologic and pathologic processes. Metal-catalyzed oxidative modification usually causes inactivation of enzymes and the appearance of carbonyl groups in amino acid side chains of the protein. We describe use of
Thapakorn Tree-Udom et al.
ACS applied materials & interfaces, 7(43), 23993-24000 (2015-10-16)
Although computer simulation and cell culture experiments have shown that elongated spherical particles can be taken up into cells more efficiently than spherical particles, experimental investigation on effects of these different shapes over the particle-membrane association has never been reported.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D193801-100G | 04061831835724 |
| 07985-5G | 04061832531328 |
| 07985-1G | 04061826660836 |