HCCB4MAG-58K

MILLIPLEX^® Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• species reactivity: human
• Quality Level: 200
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 80-110%; standard curve range: 0.1-100 ng/mL; (L1CAM); standard curve range: 0.1-100 ng/mL; (Mesothelin); standard curve range: 0.3-250 ng/mL; (Hepsin); standard curve range: 1.4-1,000 ng/mL; (CD44); standard curve range: 1.4-1,000 ng/mL; (Transglutaminase 2 (TGM2)); standard curve range: 13.7-10,000 pg/mL; (EpCAM); standard curve range: 13.7-10,000 pg/mL; (Midkine); standard curve range: 2.7-2,000 ng/mL; (ALDH1A1); standard curve range: 20.6-15,000 pg/mL; (Carbonic Anhydrase IX (CA9)); standard curve range: 27.4-20,000 pg/mL; (Kallekrein-6); inter-assay cv: <10%; intra-assay cv: <20%
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

MILLIPLEX^® Human Circulating Cancer Biomarker Panel 4 is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), and Transglutaminase 2 (TGM2). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

MILLIPLEX^® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.

Application

• Analytes: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), Transglutaminase 2 (TGM2)
• Recommended Sample Type: Human serum, plasma or tissue/cell/tumor culture supernatant
• Recommended Sample Dilution: 25 μL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• NOTE: min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL
• Research Category: Cancer
• Research Subcategory: Immune Response, Alzheimer′s Disease, Inflammation, Oncology, Tumor Markers

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable