CC130

Human Vitronectin

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM^®

• Synonym(s): S-protein, Serum-spreading factor, V75
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75
• Biological source: Escherichia coli, human
• Form: liquid
• Technique(s): cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable
• Concentration: 0.5 mg/mL
• Assay: ≥92% (SDS-PAGE)

Properties

• Product Name: PluriSTEM-XF^® Recombinant Vitronectin, Human pluripotent stem cell culture.
• Quality Segment: 100
• biological source: Escherichia coli, human
• assay: ≥92% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: PluriSTEM^®
• concentration: 0.5 mg/mL
• technique(s): cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable
• input: sample type: human embryonic stem cell(s)
• UniProt accession no.: P04004
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Application

Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion

Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Physical form

Product is supplied in a 20 mM pH 8.0 Tris-HCl buffer with a proprietary formulation of sucrose, EDTA, arginine, and DTT.

Preparation Note

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. Product has been 0.2 μm filtered.

Analysis Note

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Storage Class: 12 - Non Combustible Liquids