calreticulin; does not cross react with other endoplasmic reticulum proteins
Immunogen
peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin
Application
Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB.
Research Sub Category PI3K, Akt, & mTOR Signaling
Enzymes & Biochemistry
Quality
routinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts
Target description
60kDa
Linkage
Replaces: MABT145
Physical form
0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide.
Format: Purified
Analysis Note
Control Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Integrins mechanically link the cytoskeleton to the extracellular matrix in cardiac myocytes and are thereby involved in mechanotransduction. Integrins appear to be necessary for cardiac myocyte hypertrophy. To determine the effect of increased integrin ligation and signaling on adult cardiac
The Journal of cell biology, 138(3), 547-557 (1997-08-11)
We have isolated and characterized a 12-kb mouse genomic DNA fragment containing the entire calreticulin gene and 2.14 kb of the promoter region. The mouse calreticulin gene consists of nine exons and eight introns, and it spans 4.2 kb of
Endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression.
Michalak, M, et al.
The Journal of Biological Chemistry, 271, 29436-29445 (1996)
Calreticulin inhibits glucocorticoid- but not cAMP-sensitive expression of tyrosine aminotransferase gene in cultured McA-RH7777 hepatocytes.
Burns, K, et al.
Molecular and Cellular Biochemistry, 171, 37-43 (1997)
Myxoid liposarcoma (MLS) is a mesenchymal malignancy. To identify innovate seeds for clinical applications, we examined the proteomes of primary tumor tissues from 10 patients with MLS with different statuses of postoperative metastasis. The protein expression profiles of tumor tissues
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