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T2397

Sigma-Aldrich

Tyrode′s Salts

With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Tyrode′s buffer

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

quality

With sodium bicarbonate

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 1.0 g/L (Dextro

Dextro)
NaHCO3: 1.0 g/L

shipped in

ambient

General description

Tyrode′s Salts solution is designed to work with mammalian cells. This balanced salt solution can be used for diluting protein components of media of natural origin. Balanced Salt solution plays a role in providing a buffer system to maintain the medium in optimum pH range (7.2-7.6), irrigating and diluting while maintaining the intra- and extracellular osmotic balance. It facilitates the maintenance of normal cell metabolism by giving water and bulk inorganic ions. Balanced Salt solution provides the energy source for cell metabolism by supplying nutrients.

Application

Tyrode′s Salts has been used:
  • to incubate sperms collected from cauda epididymis of rat, mouse, guinea pig, golden hamster and rabbit
  • for the transportation of lower uterine segment (LUS) strips from women
  • for the transportation of Cor.4U human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Songying Cao et al.
Cell death & disease, 11(10), 859-859 (2020-10-17)
During mouse embryonic development, vasculogenesis initially occurs in the yolk sac, preceding neurulation. Our previous studies have demonstrated that maternal diabetes induces embryonic vasculopathy at early embryonic developmental stage by suppressing the expression of vascular growth factors including BMP4 (bone
M Joseph Costello et al.
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The unique cellular organization and transparent function of the ocular lens depend on the continuous differentiation of immature epithelial cells on the lens anterior surface into mature elongated fiber cells within the lens core. A ubiquitous event during lens differentiation
Wenlong Zhao et al.
Journal of cellular and molecular medicine, 22(3), 1755-1768 (2017-11-24)
Outer dense fibers (ODFs), as unique accessory structures in mammalian sperm, are considered to play a role in the protection of the sperm tail against shear forces. However, the role and relevant mechanisms of ODFs in modulating sperm motility and
Penghua Yang et al.
Nature communications, 10(1), 282-282 (2019-01-19)
Failure of neural tube closure results in severe birth defects and can be induced by high glucose levels resulting from maternal diabetes. MARCKS is required for neural tube closure, but the regulation and of its biological activity and function have
Ivan Kopljar et al.
Journal of pharmacological and toxicological methods, 91, 80-86 (2018-02-09)
Calcium-based screening of hiPS-CMs is a useful preclinical safety evaluation platform with the ability to generate robust signals that facilitates high-throughput screening and data analysis. However, due to the potential inherent toxicities, it is important to understand potential effects of

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