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R8781

Sigma-Aldrich

Rapamycin

from Streptomyces hygroscopicus, ≥95% (HPLC), solution, mTOR inhibitor

Synonym(s):

23,27-Epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine solution

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About This Item

Empirical Formula (Hill Notation):
C51H79NO13
Molecular Weight:
914.17
EC Number:
200-664-3
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Rapamycin, Ready Made Solution, 2.5 mg/mL in DMSO (2.74 mM), from Streptomyces hygroscopicus

biological source

Streptomyces hygroscopicus

Quality Level

300

vapor pressure

0.56 hPa ( 20 °C)

Assay

≥95% (HPLC)

form

solution

autoignition temp.

301 °C

concentration

2.5 mg/mL in DMSO (2.74 mM)

color

colorless to yellow

antibiotic activity spectrum

fungi
yeast

Mode of action

enzyme | inhibits

storage temp.

−20°C

Related Categories

General description

Rapamycin is a macrocyclic triene antibiotic possessing potent immunosuppressant and anticancer activity. It forms a complex with FKBP12 that binds to and inhibits the molecular target of rapamycin (mTOR). mTOR is a member of the phosphoinositide kinase-related kinase (PIKK) family that enhances cellular proliferation via the phosphoinositol 3-kinase/Akt signaling pathway. Inhibition of this pathway by rapamycin blocks downstream elements that result in cell cycle arrest in G1. The effectors of mTOR action include 4EBP1 and S6K1.
Rapamycin, an mTOR inhibitor, is derived from Streptomyces hygroscopicus. It can inhibit viral replication. This natural anti-fungal antibiotic is a proven anti-aging agent. Rapamycin significantly inhibits mTORC1 regardless of age.

Application

Rapamycin has been used:
  • as a supplement in mTeSR1 medium to culture induced pluripotent stem cell (iPSC) colonies for induction of autophagy signaling
  • to treat the Drosophila S2 cells at 65-70% confluence,
  • to measure protein synthesis
  • to stimulate autophagy in ovarian cancer cells

Packaging

200uL

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Keep container tightly closed in a dry and well-ventilated place. Store under inert gas. Is Hygroscopic.
Rapamycin has been expertly reviewed and recommended by the Chemical Probes Portal. For more information, please visit the Rapamycin probe summary on the Chemical Probes Portal website.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modulating eIF6 levels unveils the role of translation in ecdysone biosynthesis during Drosophila development
Arianna R, et al.
Developmental Biology, 455, 100-111 (2019)
Autophagy-dependent toxicity of amino-functionalized nanoparticles in ovarian cancer cells
Christian S, et al.
Journal of materials chemistry. B, 7, 5376-5391 (2019)
Rapamycin for longevity: opinion article
Blagosklonny, Mikhail V
Aging (Albany. NY.), 11, 8048-8048 (2019)
Yolanda T Chong et al.
Cell, 161(6), 1413-1424 (2015-06-06)
Proteomics has proved invaluable in generating large-scale quantitative data; however, the development of systems approaches for examining the proteome in vivo has lagged behind. To evaluate protein abundance and localization on a proteome scale, we exploited the yeast GFP-fusion collection
Oriana Trubiani et al.
Stem cell research & therapy, 7, 1-1 (2016-01-06)
Multiple sclerosis is a demyelinating disease mostly of autoimmune origin that affects and damages the central nervous system, leading to a disabling condition. The aim of the present study was to investigate whether administration of mesenchymal stem cells from human
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Bioactive small molecules for immune system signaling target identification/validation and antibiotics, antivirals, and antifungals offered.

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