O4878

Oxaloacetate Decarboxylase from Pseudomonas sp.

lyophilized powder, ≥100 units/mg solid

• Synonym(s): OAD
• CAS Number: 9024-98-0
• Enzyme Commission number: 4.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131775
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥100 units/mg solid
• storage temp.: −20°C

Description

General description

Oxaloacetate decarboxylase or OAD functions as a Na pump in anaerobic bacteria. It is a membrane protein consisting of three subunits, α, β and γ with the α subunit containing the carboxylase activity.

Application

Oxaloacetate Decarboxylase from Pseudomonas sp. has been used in the digestion of the low molecular weight (LMW) human milk fraction (5kF fraction) and as a positive control for deciphering C. thermocellum oxaloacetate decarboxylase activity.

Oxaloacetate decarboxylase has been used in a study to assess turnover and accessibility of a reentrant loop of the Na(+)-glutamate transporter GltS. It has also been used in a study to investigate fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources.

Biochem/physiol Actions

Oxaloacetate Decarboxylase catalyzes the decarboxylation of oxaloacetate and requires manganese and magnesium for its activity. It is associated with a wide vareity of Gram-negative bacteria.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate to pyruvate and CO₂ per min at pH 8.0 at 25 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves