Skip to Content
Merck
All Photos(2)

Documents

M2003

Sigma-Aldrich

MTT Formazan

powder

Synonym(s):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Empirical Formula (Hill Notation):
C18H17N5S
CAS Number:
57360-69-7
Molecular Weight:
335.43
MDL number:
MFCD00080731
UNSPSC Code:
12171500
PubChem Substance ID:
24896591
NACRES:
NA.47

Assay

≥97% (TLC)

Quality Level

200

form

powder

color

purple

solubility

acetone: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

Looking for similar products? Visit Product Comparison Guide

Application

MTT Formazan has been used to measure cell viability.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Apoptosis in Neurobiology, Frontiers in Neuroscience (1998)
Onofrio Zirafi et al.
Science translational medicine, 6(262), 262ra157-262ra157 (2014-11-14)
Topically applied microbicides potently inhibit HIV in vitro but have largely failed to exert protective effects in clinical trials. One possible reason for this discrepancy is that the preclinical testing of microbicides does not faithfully reflect the conditions of HIV
Combination treatment with ethyl pyruvate and IGF-I exerts neuroprotective effects against brain injury in a rat model of neonatal hypoxic-ischemic encephalopathy.
Rong Z, et. al.
International Journal of Molecular Medicine, 36, 195-195 (2015)
Sukant Garg et al.
Marine drugs, 17(6) (2019-06-15)
Fucoxanthin is commonly found in marine organisms; however, to date, it has been one of the scarcely explored natural compounds. We investigated its activities in human cancer cell culture-based viability, migration, and molecular assays, and found that it possesses strong
Xin Rui et al.
Journal of cellular and molecular medicine, 23(2), 865-876 (2018-11-06)
Long noncoding RNAs (lncRNAs) have been proved to play important roles in carcinogenesis and development of numerous cancers, but their biological functions in bladder cancer remain largely unknown. In this study, a novel lncRNA termed GAS6-AS2 were primary identified, and
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service