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J774.2 Cell Line from mouse

85011428, mouse blood

Synonym(s):

J774.2 Cells

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About This Item

UNSPSC Code:
41106514

product name

J774.2 Cell Line from mouse, BALB/C monocyte macrophage, 85011428

biological source

mouse blood

growth mode

Semi-adherent

karyotype

Not specified

products

Not specified

receptors

Immunoglobulin, complement

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse BALB/c monocyte macrophage

Cell Line Description

Recloned from J774.1 original ascites and solid tumour. Produces IL-1. Cell line J774.2 was recloned from the original ascites and solid tumor J774.1, and produces interleukin-1 (IL-1).

Application

J774.2 Cell Line from mouse has been used:
  • to study the effect of the growth hormone secretagogue peptide A233 on J774.2 cell line
  • to study the ability of Mycobacterium tuberculosis clinical isolates to penetrate and grow inside the J774.2 cell line
  • to study the effect of ivy leaf dry extract EA 575 on the lipopolysaccharide-induced release of interleukin-6 (IL-6) from J774.2 cell line

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are semi-adherent i.e. some cells grow in suspension , some loosely attach to the surface and others flattened out and attach to the flask. They should be passaged by scraping. Cells should not be allowed to overgrow and become confluent as this can lead to a loss of the flattened adherent cell characteristic.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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