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C1154

Sigma-Aldrich

4-Chloro-7-sulfobenzofurazan ammonium salt

Powder

Synonym(s):

4-Chloro-7-sulfobenz-2-oxa-1,3-diazole ammonium salt, 7-Chloro-2,1,3-benzoxadiazole-4-sulfonic acid ammonium salt, 7-Chloro-4-benzofurazansulfonic acid ammonium salt, SBF-Chloride

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About This Item

Linear Formula:
C6H3ClN2O4S · NH3
CAS Number:
81377-14-2
Molecular Weight:
251.65
Beilstein:
5200152
MDL number:
MFCD00038995
UNSPSC Code:
12171500
PubChem Substance ID:
24892366
NACRES:
NA.47

product name

4-Chloro-7-sulfobenzofurazan ammonium salt,

Assay

>98%

Quality Level

200

form

powder

color

brown
light yellow

mp

>300 °C (lit.)

solubility

water: 50 mg/mL, clear, dark yellow

fluorescence

λex 360 nm; λem 410 nm (pH 8.5)
λex 383 nm; λem 528 nm (Reaction product)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

N.OS(=O)(=O)c1ccc(Cl)c2nonc12

InChI

1S/C6H3ClN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3

InChI key

QFHXPBLOMHGYOC-UHFFFAOYSA-N

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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X P Chen et al.
Journal of chromatography. B, Biomedical sciences and applications, 709(1), 19-25 (1998-07-08)
Fluorescent adducts of 4-chloro-7-sulphobenzofurazan with cysteine, cysteinylglycine, reduced glutathione and N-acetylcysteine were prepared. Adducts were separated by HPLC on a 3-mm Nova-Pak C18 reversed-phase column using isocratic elution with a solvent of acetonitrile-0.15 M phosphoric acid (5:95) buffered at pH
R Sutton et al.
European journal of biochemistry, 142(2), 387-392 (1984-07-16)
Bovine heart mitochondrial ATPase is inhibited after covalent modification with 4-chloro-7-nitrobenzofuroxan. The kinetics of the reaction are indistinguishable from those for the reaction of an essential tyrosine residue of the ATPase with 4-chloro-7-nitrobenzofurazan that have been described previously [Ferguson et
Ammonium 4-chloro-7-sulfobenzofurazan: a new fluorigenic thiol-specific reagent.
J L Andrews et al.
Archives of biochemistry and biophysics, 214(1), 386-396 (1982-03-01)
R M Bolton et al.
Analytical biochemistry, 216(2), 418-423 (1994-02-01)
Ammonium 4-chloro-7-sulfobenzofurazan (Sbf-Cl) is a water-soluble fluorescent reagent which is highly specific for thiol groups. We describe here a simple and sensitive fluorescence assay which is highly specific for subunit 3 of the rat glutathione S-transferases. Specific activities of isoenzymes
Bin Meng et al.
BioMed research international, 2015, 815210-815210 (2015-11-12)
This study was aimed at investigating the effects of quercetin on mRNA expression and activity of critical enzymes in homocysteine metabolism in rats fed a methionine-enriched diet. Rats were fed for 6 weeks the following diets, that is, control, 0.5%

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