A6410

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

• Synonym(s): Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
• CAS Number: 9000-95-7
• Enzyme Commission number: 3.6.1.5 (BRENDA, IUBMB)
• EC Number: 232-569-8
• MDL number: MFCD00130542
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Grade VI
• Quality Level: 200
• form: lyophilized powder
• ATPase activity: ≥200 units/mg protein
• secondary activity: ≤15 % of base activity ADPase
• mol wt: ~49 kDa by gel filtration
• composition: Protein, 30-60%
• solubility: H₂O: soluble 1.0 mg/mL
• foreign activity: Acid Phosphatase ≤2% of base activity
• storage temp.: −20°C

Description

General description

Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.

Application

Apyrase from potatoes has been used:

• to deplete ATP
• to degrade endogenous extracellular ATP
• as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Biochem/physiol Actions

Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Other Notes

This grade has an exceptionally high ATPase/ADPase ratio.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from white potato

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves