ABF32
Anti-Fibroblast-specific Protein 1 (S100A4) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, rat, human, horse
species reactivity (predicted by homology)
canine (based on 100% sequence homology), bovine (based on 100% sequence homology), equine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... S100A4(6275)
General description
S100A4, also known as Fibroblast-specific Protein 1, is a member of the Ca2+- dependent S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 is a metastasis factor that may function in motility, invasion, and tubulin polymerization. Increased expression of S100A4 has been associated with various types of cancer.
Specificity
This antibody recognizes S100A4 at the C-terminus.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human S100A4.
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected S100A4 in mouse neural stem cells.
Research Category
Stem Cell Research
Inflammation & Immunology
Stem Cell Research
Inflammation & Immunology
Research Sub Category
Ion & Transport Channels
Inflammation & Autoimmune Mechanisms
Ion & Transport Channels
Inflammation & Autoimmune Mechanisms
This Anti-Fibroblast-specific Protein 1 (S100A4) Antibody is validated for use in WB, IC for the detection of Fibroblast-specific Protein 1 (S100A4).
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.
Target description
~12 kDa observed
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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International journal of cancer, 142(10), 2056-2067 (2017-12-22)
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