567375

Smac-N7 Peptide, Cell-Permeable

The Smac-N7 peptide is modified to be cell permeant by linking the lysine carboxyl terminal to the arginine of Antennapedia homeodomain 16-mer peptide via a proline linker.

• Synonym(s): Smac-N7 Peptide, Cell-Permeable, AVPIAQKPRQIKIWFQNRRMKWKK
• Empirical Formula (Hill Notation): C₁₄₂H₂₃₂N₄₄O₂₉S
• Molecular Weight: 3051.70
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

The Smac-N7 peptide (Cat. No. 567370) is modified to be cell permeant by linking the lysine carboxyl terminal to the arginine of Antennapedia homeodomain 16-mer peptide via a proline linker.

The Smac-N7 peptide (Cat. No. 567370) where the lysine carboxy terminal is linked to the arginine of Antennapedia homeodomain 16-mer peptide (underlined) via a proline linker to make it cell-permeable. Smac-N7 peptide contains the N-terminal seven residues of Smac and promotes the activation of procaspase-3 (~10 µM). Also, promotes the catalytic activity of caspase-9 in the presence of XIAP.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Promotes procaspase-3 activation

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Ala-Val-Pro-Ile-Ala-Gln-Lys-Pro-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Srinivasula, S.M., et al. 2001. Nature410, 112.
Chai, J., et al. 2000. Nature406, 855.
Liu, Z., et al. 2000. Nature408, 1004.
Srinivasula, S.M., et al. 2000. J. Biol. Chem.275, 36152.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable