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  • Expression of coat proteins changes during postnatal development in selected areas of the rat brain.

Expression of coat proteins changes during postnatal development in selected areas of the rat brain.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience (2012-02-07)
Janina Borgonovo, Pablo Capella, Alicia Seltzer, Miguel A Sosa
ABSTRACT

It is well known that clathrin-mediated endocytosis is crucial for the normal functioning and integrity of neurons in the central nervous system. In this study we attempted to correlate the expression of coat proteins with development in different areas of rat brain. By Western blot, we studied the expression of AP-2, GGA1 and GGA2 in striatum, cerebellum, brain stem, cerebral cortex and hippocampus of newborn rats and during post-natal development; 5, 15, 30, 60, 90 or 150 days after birth. We observed that the expression of the α2 subunit of AP-2 increased substantially between the 15th and 30th day after birth in all areas studied, excepting the cerebellum and cortex. On the other hand, the expression of the α1 subunit does not change significantly during the development in any of the areas under study. We also noted that the expression of the μ2 subunit did not follow the pattern of α2 during development. In general terms, the expression of GGA1 and GGA2 followed a similar pattern to that of AP-2, although these proteins increased significantly in the cerebral cortex from the 15th day after birth. Moreover, presenilin-1, a protein associated with aging and neurodegeneration, shows an expression pattern similar to coat proteins in the striatum and cortex. These results suggest that proteins that conform the intracellular transport machinery in the brain cells seems to accompany development, according to the maturation of the different brain areas.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Goat IgG (whole molecule)−Agarose antibody produced in rabbit, IgG fraction of antiserum, PBS suspension
Sigma-Aldrich
Avidin–Peroxidase, buffered aqueous solution
Sigma-Aldrich
Anti-Presenilin-1 (S182) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution