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813071

TSKgel® Amide-80 (5 µm) HPLC Columns

L x I.D. 25 cm × 4.6 mm, carbamoyl phase

Synonym(s):

TSKgel® Amide-80, 25 cm x 4.6 mm ID, 5 μm

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
Pricing and availability is not currently available.

Product Name

TSKgel® Amide-80 HPLC Column, phase carbamoyl, L × I.D. 25 cm × 4.6 mm, 5 μm particle size

material

stainless steel

Agency

suitable for USP L68

product line

TSKgel®

manufacturer/tradename

Tosoh 13071

parameter

0-100% organic solvent
10-80 °C temperature
150 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

450 m2/g

matrix

silica particle platform

matrix active group

carbamoyl phase

particle size

5 μm

pore size

100 Å

operating pH

2.0-7.5

separation technique

hydrophilic interaction (HILIC)

General description

TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

Physical form

Shipped in 85% acetonitrile - 15% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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