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796-0657

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

application for HPLC

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About This Item

description

Separated on Discovery® BIO PolyMA-WAX

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
detector: UV, 280 nm
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Trypsin Inhibitor

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