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58220C40

Supelco

SUPELCOSIL™ LC-8 (5 µm) HPLC Columns

L × I.D. 15 cm × 4 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL™ LC-8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

SUPELCOSIL™

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

packaging

1 ea of

extent of labeling

6.0% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4 mm

surface area

170 m2/g

surface coverage

surface coverage 3.2 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

C8 (octyl) phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
291178-01
291050-01
288864-01
284607-01
284601-01

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Thomas Borch et al.
Journal of chromatography. A, 1022(1-2), 83-94 (2004-02-03)
Explosives such as 2,4,6-trinitrotoluene (TNT), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) are widely distributed environmental contaminants. Complete chromatographic separation is necessary in order to accurately determine and quantify explosives and their degradation products in environmental samples and in (bio)transformation studies. The
F Belal et al.
Journal of pharmaceutical and biomedical analysis, 24(3), 335-342 (2001-02-24)
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of ramipril and hydrochlorothiazide in their dosage forms. Acetonitrile: sodium perchlorate solution (0.1 M) adjusted to pH 2.5+/-0.2 with phosphoric acid (46:54 v/v), was used as
M Molíková et al.
Journal of chromatography. A, 1217(8), 1305-1312 (2010-01-12)
The availability of ionic liquids (ILs) in wide areas of application often results in the requirement on their determination. The attention is also often focused on the knowledge of hydrophobicity as it plays a key role in the biological effects
L Dal Bo et al.
Journal of chromatography. B, Biomedical applications, 665(2), 404-409 (1995-03-24)
A simple HPLC method has been developed for the determination of ticlopidine in human plasma. Plasma samples were buffered at pH 9 and extracted with n-heptane-isoamyl alcohol (98.5:1.5, v/v). Imipramine was used as internal standard. Chromatography was performed isocratically with
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
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