X0629

Anti-XRCC1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-X-ray repair complementing defective repair in Chinese hamster cells 1
• MDL number: MFCD01865396
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 85 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton; western blot: 0.5-1 μg/mL using A431 cell lysates
• UniProt accession no.: P18887
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... XRCC1(7515) ; mouse ... Xrcc1(22594) ; rat ... Xrcc1(84495)

Description

General description

X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein. It is considered as a DNA repair gene.

Immunogen

synthetic peptide corresponding to amino acids 615-633 of human XRCC1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is identical in mouse and rat.

Application

Anti-XRCC1 antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• indirect immunofluorescence and telomere FISH (TEL-FISH)
• protein detection
• western analysis

Biochem/physiol Actions

X-ray repair cross-complementing group 1 (XRCC1) is a molecular scaffold protein that coordinates the assembly of repair complexes at damaged sites. XRCC has been shown to physically interact with several enzymes known to be involved in the repair of SSBs, including DNA ligase IIIa, DNA polymerase b, APE1, polynucleotide kinase (PNK), poly (ADP-ribose), polymerases 1 and 2 (PARP-1 and 2) and others. It participates in the DNA base excision repair (BER) pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves