W0138
Western Blocker™ Solution
for HRP detection systems
Synonym(s):
Western blot blocking solution, blocking buffer
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About This Item
UNSPSC Code:
41105329
NACRES:
NA.25
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![Western Blocking Reagent, Solution solution, sufficient for 10 blots (11921673001 [100 cm2]), sufficient for 60 blots (11921681001 [100 cm2])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
General description
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Application
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Features and Benefits
- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
Legal Information
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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