SAB4200394

Anti-Nestin (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-NES
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~300 kDa
• species reactivity: mouse, rat
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.5 mg/mL
• technique(s): indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells; western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin.
• UniProt accession no.: P21263
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Nes(18008) ; rat ... Nes(25491)

Description

General description

Nestin/NES belongs to the intermediate filament (IF) protein family. Its expression is found in immature neuroepithelial and progenitor cell types. This 240 kDa protein contains the shortest head domain (N-terminus) and a long tail domain (C-terminus). Nestin is mapped to human chromosome 1q23.1.

Immunogen

synthetic peptide corresponding to a sequence at the C- terminus of mouse nestin, conjugated to KLH. The sequence is identical in rat nestin.

Application

Anti-Nestin (C-terminal) antibody has been used in immunostaining.

Biochem/physiol Actions

Nestin/NES plays a major role in the modulation of assembly and disassembly of intermediate filaments, which in turn help in the remodeling of the cell along with other structural proteins.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable