SAB2700730

Monoclonal Anti-TET1 antibody produced in mouse

clone GT1462, affinity isolated antibody, buffered aqueous solution

• Synonym(s): CXXC6, FLJ10839, FLJ41442, TET1
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: GT1462, monoclonal
• form: buffered aqueous solution
• species reactivity: human, mouse
• technique(s): ChIP: suitable; immunofluorescence: suitable; immunohistochemistry: 1:100-1:1000; immunoprecipitation (IP): suitable; western blot: 500-3000
• isotype: IgG2
• NCBI accession no.: NP_085128
• UniProt accession no.: Q8NFU7
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TET1(80312)

Description

General description

Tet methylcytosine dioxygenase 1 (TET1) is a protein that belongs to the TET family and contains a zinc-binding CXXC domain. It is expressed in embryonic stem cells. The TET1 gene is localized on human chromosome 10q21.3.

Application

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, IP: 1:100-1:500, WB: 1:500-1:3000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Biochem/physiol Actions

Tet methylcytosine dioxygenase 1 (TET1) functions as a 5-methylcytosine (5mC) hydroxylase. It converts 5-methylcytosine to 5-hydroxymethylcytosine, which is crucial for active DNA demethylation in the adult brain. The protein has been linked to the progression of colorectal cancer.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Phosphate-buffered saline, no preservative added.

Storage and Stability

Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Other Notes

For in vitro laboratory use only. Not for any clinical, therapeutic or diagnostic use in humans or animals. Not for animal or human consumption.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H412
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable