indirect immunofluorescence: 5-10 μg/mL using human HeLa cells western blot (chemiluminescent): 1-2 μg/mL using whole extracts of mouse NIH-3T3 and rat ROS cells
Sec23 has two isoforms, Sec23A and Sec23B. Sec23A is mainly found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles.
Sec23A (Sec23 homolog A) is a human Sec23 paralog, that is a GTPase-activating protein. It is a fundamental constituent of the coat protein II complex. It is located on human chromosome 14q21.
Immunogen
synthetic peptide corresponding to amino acid residues 1-18 of human Sec23A with C-terminal added cysteine conjugated to KLH. The corresponding sequence is identical in rat and mouse Sec23A and differs by one amino acid in human and mouse Sec23B.
Application
Anti-Sec23 antibody has been used in immunoblotting, in western blot analysis, immunofluorescence.
Biochem/physiol Actions
Sec23A (Sec23 homolog A) plays an important role in protein trafficking between the endoplasmic reticulum and Golgi apparatus. It also participates in anti-tumorigenesis. Suppressing the expression of Sec23A induces multiplication, migration, and invasion of CRC cells (colorectal cancer). Mutation in SEC23A gene results in cranio-lenticulo-sutural dysplasia.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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