The GenElute HP Plasmid Kits are much faster than other commercially available kits. A filter syringe is used for preparing the cleared lysate, which replaces a lengthy centrifugation step. These kits also offer the option of using a vacuum or spin purification format rather than a gravity drip to bind, wash and elute the plasmid. In addition, samples are eluted in Tris buffer so there are no alcohol precipitations required.
NA0200
GenElute™ HP Plasmid Midiprep Kit
sufficient for 25 purifications
Synonym(s):
GenElute™ HP Plasmid Midiprep Kit, HP midiprep kit, fast midiprep kit, fast plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation kit, Gen Elute
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.52
Pricing and availability is not currently available.
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usage
sufficient for 25 purifications
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
greener alternative category
, Aligned
storage temp.
15-25°C
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General description
The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Features and Benefits
- From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
- Up to 350 μg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- No phenol/chloroform extraction or alcohol precipitation required
- Contains fewer plastic components, reducing the amount of waste
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
3 - Flammable liquids
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Related Content
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
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How do the GenElute HP Plasmid Kits differ from other plasmid purification systems?
1 answer-
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Which format gives a better yield, vacuum or spin?
1 answer-
There is no difference in plasmid recovery when using either format.
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Do the binding columns fit on other manufacturers’ manifolds?
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Yes. The columns fit on all standard vacuum manifolds.
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What is the expected yield of plasmid DNA that you can isolate from these kits?
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Yields can reach as high as 350 mg for a midiprep and 1.2 mg for a maxiprep. As with any protocol, lower yields can be expected with low copy plasmids.
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Is plasmid DNA isolated with this kit suitable for use in transfection?
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Yes. However, if you are currently required to use an endofree plasmid purification kit, we recommend using the GenElute HP Endotoxin-free Kit.
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How long does it take to isolate plasmid using the vacuum format?
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A midiprep or maxiprep requires only 30 minutes to complete.
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