Skip to Content
Merck
All Photos(3)

Key Documents

MBD0048

Sigma-Aldrich

Enterococcus faecium FISH probe - Cy3

Probe for fluorescence in situ hybridization (FISH), 20µM in water

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.24

Quality Level

technique(s)

FISH: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells. Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples. FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood and tissue), microbial ecology (solid biofilms, aquatic systems) and plants. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest.
Enterococcus faecium probe specifically recognizes E. faecium cells,. E. faecium, a gram-positive bacteria can cause various complicated infectionsincluding endocarditis, urinary tract infections, prostatitis, intra-abdominalinfection, cellulitis, and wound infection and concurrent bacteremia. Studies have shown that E. faecium is highly resistant to multiple antibiotics and that vancomycin-resistant Enterococcus faecium (VRE) can be asymptomatically carried by healthy people, which can hamper hospitals’ attempts to control the spread of the bacteria. Over the past two decades, Enterococcus faecium has emerged as a leading cause of multidrug-resistant enterococcal infection in the United States. E. faecium has a high antibiotic-resistance with more than half of its pathogenic isolates expressing resistance to vancomycin, ampicillin, and high-levels of aminoglycosides.

Application

Enterococcus faecium FISH probe - Cy3 is suitable to use as a probe for fluorescence in situ hybridization (FISH) to recognize Enterococcus faecium cells in clinical samples.

Features and Benefits

  • Visualize, identify, and isolate E. faecium cells.
  • Observe native E. faecium cell populations in diverse microbiome environments.
  • Specific, sensitive, and robust identification of E. faecium in bacterial mixed population.
  • Specific, sensitive, and robust identification even when E. faecium is in low abundance in the sample.
  • FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
  • Provides information on E. faecium morphology and allows to study biofilm architecture.
  • Identify E. faecium in clinical samples such as tumor and brain tissues (for example in formalin-fixed paraffin-embedded (FFPE) samples), saliva and oral cavity and medical equipment such as dental implants and voice prostheses.
  • The ability to detect E. faecium in its natural habitat is an essential tool for studying host-microbiome interaction.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization
Wellinghausen, N., Bartel, M., Essig, A. & Poppert, S
Journal of Clinical Microbiology (2007)
Genotyping and preemptive isolation to control an outbreak of vancomycin-resistant Enterococcus faecium
Mascini, E. M. et al
Clinical Infectious Diseases, 739-46, 42-42 (2006)
Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay
Peters, R. P. H. et al
Journal of Clinical Microbiology, 44, 4186-4188 (2006)
NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, 2006-200
Hidron, A. I. et al
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 996-1011, 29-29 (2008)
Cesar A Arias et al.
Nature reviews. Microbiology, 10(4), 266-278 (2012-03-17)
The genus Enterococcus includes some of the most important nosocomial multidrug-resistant organisms, and these pathogens usually affect patients who are debilitated by other, concurrent illnesses and undergoing prolonged hospitalization. This Review discusses the factors involved in the changing epidemiology of

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service