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HPA008599

Sigma-Aldrich

Anti-TAF4 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-TAF(II)135 antibody produced in rabbit, Anti-TAFII-130 antibody produced in rabbit, Anti-TAFII-135 antibody produced in rabbit, Anti-TAFII130 antibody produced in rabbit, Anti-TAFII135 antibody produced in rabbit, Anti-TBP-associated factor 4 antibody produced in rabbit, Anti-Transcription initiation factor TFIID 135 kDa subunit antibody produced in rabbit, Anti-Transcription initiation factor TFIID subunit 4 antibody produced in rabbit

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
HPA008599
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

VLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCK

UniProt accession no.

O00268

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TAF4(6874)

General description

TAF4 (TATA-box binding protein associated factor 4) is a component of transcription factor complex TFIID, which forms an essential part of the transcriptional machinery. TFIID contains TATA-binding protein (TBP) and 14 TAFs. It contains two copies of TAF4. The N-terminal of TAF4 contains a conserved glutamine rich domain. Its core is composed of co-activator TAF4-homology ETO-TAFH/CRI/NHR1 (TAFH) domain, and its C-terminal contains a histone-like (CRII) domain.

Immunogen

Transcription initiation factor TFIID subunit 4 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

TAF4 (TATA-box binding protein associated factor 4) is responsible for maintaining the structural stability of the TFIID complex. It mediates interactions with CREB (cAMP response element-binding) and Sp1 (specificity protein) proteins, and as it is a part of the Wnt signaling, it plays a role in cell fate determination and differentiation and development. Its controlled degradation is linked to proper male germ-cell differentiation, and TAF4b is essential for the development of oocytes and spermatogenesis. It interacts with activation function-2 (AF-2), and induces the transcription of retinoic acid, vitamin D3 and thyroid hormone receptors. It is also involved in the regulation of neurongeneis and neuronal differentiation, where it interacts with TP53 and noncanonical Wnt signaling.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST70914

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jekaterina Kazantseva et al.
Journal of molecular neuroscience : MN, 55(1), 160-166 (2014-04-04)
Expression of general transcription factor and co-activator TAF4 varies during development and in the processes of cell differentiation with suggested connection to neurodegenerative diseases. Here, we show that expression of TAF4 alternative splice variants is different in various regions of
G Mengus et al.
Genes & development, 11(11), 1381-1395 (1997-06-01)
We report for the first time the cloning of a complete cDNA encoding the human TFIID subunit hTAF(II)135 (hTAF(II)130). Full-length hTAF(II)135 comprises 1083 amino acids and contains two conserved domains present also in dTAF(II)110 and hTAF(II)105. We show that expression
Jekaterina Kazantseva et al.
PloS one, 8(10), e74799-e74799 (2013-10-08)
Transcription factor IID (TFIID) activity can be regulated by cellular signals to specifically alter transcription of particular subsets of genes. Alternative splicing of TFIID subunits is often the result of external stimulation of upstream signaling pathways. We studied tissue distribution

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