Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

A2055

Sigma-Aldrich

ACCUSPIN™ Tubes Sterile, 50 mL Capacity

radiation sterilized tube fitted with a high density polyethylene barrier

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.

Quality Level

200

sterility

sterile; irradiated

form

tubes

application(s)

hematology
histology

storage temp.

15-25°C

Related Categories

General description

ACCUSPIN™ Tubes are polypropylene radiation sterilized tube fitted with a high density polyethylene barrier. Each tube will accept 15 mL of the density gradient.

Application

ACCUSPIN™ Tubes Sterile, 50 mL Capacity is intended for use with the density gradient separation medium Histopaque™ -1077:


  • in the isolation of lymphocytes and other mononuclear cells.
  • isolation of cord blood–derived endothelial progenitor cells.[1]
  • isolate mononuclear cell layer from bone marrow samples.
  • suitable for the purification of human bone marrow aspirates for the investigation of the therapeutic value of BET inhibitors for acute myeloid leukemia.

Principle

Following the addition of Histopaque-1077 to theACCUSPIN Tube, a brief centrifugation places theHistopaque-1077 below the frit. The blood sample canbe added to the top chamber of the tube without risk ofmixing with the Histopaque-1077 in the lower chamberunder the frit. On subsequent centrifugation the wholeblood descends through the frit to contact with theHistopaque-1077. The elements of greater densitydisplace a volume of Histopaque-1077 above the fritgiving a clear separation of the blood components. Theerythrocytes aggregate and the granulocytes becomeslightly hypertonic, increasing their sedimentation rate,resulting in pelleting at the bottom of the ACCUSPINTube. Lymphocytes and other mononuclear cells, e.g.,monocytes, remain at the plasma/Histopaque-1077interface. This dense band of mononuclear cells maybe collected by pouring off the contents of the upperchamber or by means of a pipette. Erythrocytecontamination is avoided due to the barrier between thechambers.

Quantity

Package contains 10 tubes

Legal Information

Accuspin is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000411992
0000411992
0000367710
0000357493
0000293741

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

S A Mireku et al.
Scientific reports, 7(1), 14296-14296 (2017-11-01)
Bacterial ABC importers catalyze the uptake of essential nutrients including transition metals and metal-containing co-factors. Recently, an IgG antibody targeting the external binding protein of the Staphylococcus aureus Mn(II) ABC importer was reported to inhibit transport activity and reduce bacterial
Kara S Cox et al.
mAbs, 8(1), 129-140 (2015-10-23)
Monitoring antigen-specific memory B cells and the antibodies they encode is important for understanding the specificity, breadth and duration of immune response to an infection or vaccination. The antibodies isolated could further help design vaccine antigens for raising relevant protective
Samuel J S Rubin et al.
Nature communications, 10(1), 2686-2686 (2019-06-21)
Inflammatory bowel disease (IBD) includes Crohn's disease and ulcerative colitis. Each disease is characterized by a diverse set of potential manifestations, which determine patients' disease phenotype. Current understanding of phenotype determinants is limited, despite increasing prevalence and healthcare costs. Diagnosis
Aimin Tang et al.
Nature communications, 10(1), 4153-4153 (2019-09-14)
Respiratory syncytial virus (RSV) infection is the leading cause of hospitalization and infant mortality under six months of age worldwide; therefore, the prevention of RSV infection in all infants represents a significant unmet medical need. Here we report the isolation
Juan M Melero-Martin et al.
Methods in enzymology, 445, 303-329 (2008-11-22)
Rapid and complete vascularization of ischemic tissues and thick engineered tissues is likely to require vasculogenesis. Therefore, the search for clinically relevant sources of vasculogenic cells and the subsequent development of experimental models of vasculogenesis is of utmost importance. Here
View All Related Papers

Articles

Magnetic Nanoparticles for Cancer Theranostics

The recent emergence of a number of highly functional nanomaterials has enabled new approaches to the understanding, diagnosis, and treatment of cancer.

Magnetic Nanoparticles for Cancer Theranostics

The recent emergence of a number of highly functional nanomaterials has enabled new approaches to the understanding, diagnosis, and treatment of cancer.

Magnetic Nanoparticles for Cancer Theranostics

The recent emergence of a number of highly functional nanomaterials has enabled new approaches to the understanding, diagnosis, and treatment of cancer.

Magnetic Nanoparticles for Cancer Theranostics

The recent emergence of a number of highly functional nanomaterials has enabled new approaches to the understanding, diagnosis, and treatment of cancer.

Questions

1–5 of 5 Questions  

  1. How should a 4-20% sucrose gradient be used to reduce platelet contamination, as recommended in the user manual?

    1 answer

    1. Sucrose exerts an osmotic pressure of 300 mOsm at a concentration of about 0.25M, which is iso-osmotic with the contents of most mammalian cells. This concentration is approximately 81.25 grams/L or 8.125%, as per protocols used to remove platelets. Recommended procedures for removing platelets:
      Perform Procedure 1077 or 1077/1119.
      Collect the cell band and layer it over a second gradient.
      A. Bottom layer = 1077-1
      B. Top layer = 8.125% sucrose solution (prepared in deionized water).
      C. Layer the cell suspension on top of the gradient formed in steps 1-2.
      D. Use a 300 g spin for 10-15 minutes.
      E. Depending upon the original volume of the cell suspension, times and speed may have to be adjusted to maximize cell recovery.
      F. Use separate tubes for mononuclear and polymorphonuclear cells.

      Helpful?

  2. Does the Accuspin Tubes Product #A2055, which are prefilled with a frit, also contain EDTA or another anticoagulant?

    1 answer

    1. None of the Accuspin spin tubes (filled or unfilled) contain any anticoagulant. It is expected that when blood is drawn, it is typically drawn into a vacuum tube that already contains an anticoagulant or it is necessary to add anticoagulant immediately after the blood draw is completed. The most common anticoagulants used are EDTA and Heparin.

      Helpful?

  3. Are Accuspin tubes compatible with blood that has been anticoagulated with ACD-A?

    1 answer

    1. Certainly, Accuspin tubes are suitable for use with any blood containing an anticoagulant, with fresher blood being preferable. Regardless of the anticoagulant used, the anticoagulant is essentially washed away during the steps to remove the density gradient from the collected white blood cells. With ACD-A, the cell band is wider at the interface and the cells are more dispersed over a larger area, possibly due to the dextrose/glucose in the formulation. When using ACD-A, a somewhat unusual cell band is produced after the tubes are removed from the centrifuge. In comparison, cell bands with heparin, EDTA, sodium citrate, and other typical anticoagulants typically provide a rather narrow cell band when viewed from the side of the Accuspin tube.

      Helpful?

  4. Is Accuspin (A2055) suitable for use with canine blood?

    1 answer

    1. The suggestion is to make a decision based on individual circumstances.If currently using a plain centrifuge tube for collecting PBMCs from canine blood using a single density gradient, the Accuspin Tubes can be used for the same collection, provided that the G force does not exceed 1000G and a single density gradient is used.

      Helpful?

  5. Can I remove the Frit after collecting the mononuclear cells to recover the granulocytes?

    1 answer

    1. When using the Accuspin System to collect Peripheral Blood Mononuclear Cells (PBMCs), there is no expectation that the neutrophils can be recovered. Although it is possible to dislodge the frit, some granulocytes will be suspended in the lower layers of the density gradient. The remaining granulocytes will appear as a gray layer on top of the packed red blood cells.

      In theory, the end-user may be able to collect this buffy coat that sits atop the packed red blood cell layer. It is recommended to either perform a lysis step to lyse the red blood cells, or run the cells back over another density gradient - such as Histopaque 11191 that has a density of 1.119 g/ml.

      Instead of performing this 2-step procedure that has not been validated, the more logical option would be to simply run the whole peripheral blood over a 2-step discontinuous gradient. Currently, Histopaque 10771 and Histopaque 11191, are offered. The Histopaque 11191 instructions for use are available in the DOCUMENTATION section in the 'More Documents' tab of the product detail page:
      https://www.sigmaaldrich.com/product/sigma/a2055#product-documentation
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/193/872/a2055pis.pdf

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service