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MABE366

Sigma-Aldrich

Anti-SNF2L Antibody, clone SNF 2C4

clone SNF 2C4, from rat

Synonym(s):

Probable global transcription activator SNF2L1, ATP-dependent helicase SMARCA1, Nucleosome-remodeling factor subunit SNF2L, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SNF 2C4, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SMARCA1(6594)

General description

Nucleosome-remodeling factor subunit SNF2L, also known as SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1 (SMARCA1), is the energy-transducing component of NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. These complexes facilitate the disruption of chromatin structure in an ATP-dependent manner. SNF2L potentiates neurite outgrowth, and may be involved in brain development by regulating En-1 and En-2 expression as well as in the development of luteal cells.

Immunogen

GST-tagged recombinant protein corresponding to human SNF2L.

Application

Detect the SNF2L protein using this Anti-SNF2L, clone SNF 2C4 validated for use in WB & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Western Blot Analysis: A representative lot was used by an an independent laboratory to detect SNF2L in untransfected HeLa whole cell extracts, but demonstrated a loss of signal in HeLa whole cell extracts transfected with siRNA. (Eckey, M., et al. (2012). Mol Cell Biol. 32(13):2359-2371).
Immunohistochemistry Analysis: A representative lot was used by an an independent laboratory to detect SNF2L in certain, normal human organ tissues. (Eckey, M., et al. (2012). Mol Cell Biol. 32(13):2359-2371).

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1.0 µg/mL of this antibody detected SNF2L in 10 µg of HeLa cell lysate.

Target description

~121 and ~123 kDa observed. Uniprot describes 5 tyrosine phosphorylation sites.

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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