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MAB16985F

Sigma-Aldrich

Anti-MCAM Antibody, clone P1H12, FITC conjugated

clone P1H12, Chemicon®, from mouse

Synonym(s):

MUC18, CD146

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

P1H12, monoclonal

species reactivity

canine, mouse, human

should not react with

rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MCAM(4162)

General description

MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).

Specificity

Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.
P1H12 reacts specifically with CD146 {MCAM, MUC18}. In blood and marrow P1H12 reacts only with endothelial cells, and has been used to detect circulating endothelial cells in human, dogs and mouse samples. It positively stains normal, primary HUVEC and MVEC cultures and the endothelial cells of all vessels in normal frozen sections of human skin, intestine, ovary tonsil, lymph node, lung, and kidney. It does not stain carcinoma cell lines HT-29 and COLO205, and M21, the T cell lines Jurkat and HuT78, fibroblasts, HL-60 or CHO cells, or EBV-transformed B cell lines, although it expected that CD146 expression is present in many tumor lines. P1H12 does not stain normal monocytes, granulocytes, red cells, platelets, T cells or B cells from marrow or peripheral blood; nor does it detect marrow megakaryocytes or the megakaryoblast line HU3. The peripheral blood cells that do stain with P1H12 also are positive for both von Willebrand Factor (vWF) and thrombomodulin (the combined expression of which is limited to endothelium), and they stain for flt and flk. In recent testing, melanoma A2058, SKMEL.3 and A375.S2 are positive, while murine melanoma M3 is negative. One smooth muscle line (HISM) was positive and one negative (TIGHA-VSMC). Human blood lymphoctes are negative with or without prior stimulation.

Immunogen

Immunization of mice with HUVEC

Application

Detect MCAM using this Anti-MCAM Antibody, clone P1H12, FITC conjugated validated for use in FC.
Immunocytochemistry: 1-10 μg/mL. Works best on EDTA or Trypsin lifted endothelial cells.

Immunohistochemistry: 1-10 μg/mL. 4% PFA for 30min RT or <2hrs @4°C. Block w/ 1%BSA/0.2%tween20/PBC for 30min. Works well in frozen tissue; fixed or unfixed.

Facs Analysis: 1-10 μg/mL

Optimal working dilutions and protocols must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Physical form

Protein A purified
Purified immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH=7.6, 15 mg/nL BSA containing 0.1% sodium azide as a preservative.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
HUVEC cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stefania Elena Navone et al.
Vascular cell, 5(1), 10-10 (2013-05-16)
Brain microvascular endothelial cells (BMVECs) constitute the primary limitation for passage of ions and molecules from the blood into the brain through the blood brain barrier. Numerous multi-step procedures for isolating and culturing BMVECs have been described. However, each one
Nadia Quirici et al.
Stem cells and development, 19(6), 915-925 (2009-11-26)
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Phase II study of Cediranib (AZD 2171), an inhibitor of the vascular endothelial growth factor receptor, for second-line therapy of small cell lung cancer (National Cancer Institute #7097).
Ramalingam, SS; Belani, CP; Mack, PC; Vokes, EE; Longmate, J; Govindan, R; Koczywas et al.
Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer null
Maria M Zanone et al.
Journal of immunology (Baltimore, Md. : 1950), 171(1), 438-446 (2003-06-21)
Numerous studies indicate that enteroviruses, such as the Coxsackievirus (CV) group, are linked to autoimmune diseases. Virus tropism and tissue access are modulated by vascular endothelial cells (ECs), mainly at the level of the microvasculature. Data on the permissiveness of
Stefania E Navone et al.
Nature protocols, 8(9), 1680-1693 (2013-08-10)
Brain microvascular endothelial cells (BMVECs) have an important role in the constitution of the blood-brain barrier (BBB). The BBB is involved in the disease processes of a number of neurological disorders in which its permeability increases. Isolation of BMVECs could

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