It is a good antibody for Western Blot for both specimen of human and mouse. But it often shows signals medium intensity, not strong enough.
V8137
Anti-V5 antibody produced in rabbit
IgG fraction of antiserum
Anti-V5 antibody produced in rabbit
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
Rabbit Anti-V5
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IP, WB
Citations:
121
Pricing and availability is not currently available.
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
technique(s)
immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells, immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells, western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.
Application
Anti-V5 antibody produced in rabbit has been used in:
- immunocytochemistry
- immunoprecipitation
- western blot
Biochem/physiol Actions
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Physical form
Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.
Preparation Note
Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form IgG fraction of antiserum | antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form affinity isolated antibody |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| technique(s) immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells, western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells, immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells | technique(s) immunoprecipitation (IP): 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates (tagged fusion proteins from transfected mammalian cell lysates), indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells, western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates | technique(s) immunoprecipitation (IP): 10 μg using mammalian cell extracts expressing VP16 fusion proteins, western blot: 1:1,000 using mammalian cell extracts expressing VP16 fusion proteins | technique(s) immunoprecipitation (IP): 5-10 μg using lysates of human Jurkat cells., western blot: 2.5-5 μg/mL using whole extracts of mouse A20 cells. |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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