SHC005V

MISSION^® pLKO.1-puro eGFP shRNA Control Transduction Particles

shRNA sequence targeting eGFP

• Synonym(s): MISSION^®, MISSION^® Control Transduction Particles
• NACRES: NA.51
• UNSPSC Code: 41106609
• Concentration: ≥1x10⁶ VP/ml (via p24 assay)
• Technique(s): capture ELISA: 10⁶ TU/mL using p24

Properties

• Quality Segment: 200
• product line: MISSION^®
• concentration: ≥1x10⁶ VP/ml (via p24 assay)
• technique(s): capture ELISA: 10⁶ TU/mL using p24
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The MISSION eGFP shRNA Control Transduction Particles contain an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761). The eGFP shRNA Control Particles are useful as a positive knockdown control in experiments using cell lines expressing eGFP.
The eGFP shRNA Control Transduction Particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-eGFP shRNA (Prod. No. SHC005). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.[1] In addition, the control transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)