SAB4200201

Anti-Cyclophilin B antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-CYP-S1, Anti-CYPB, Anti-OI9, Anti-PPIB, Anti-PPIase B, Anti-S-cyclophilin, Anti-SCYLP, Anti-cyclophilin-like protein, Anti-peptidyl-prolyl cis-trans isomerase B, Anti-peptidylprolyl isomerase B, Anti-rotamase B
• MDL number: MFCD02265740
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~20 kDa
• species reactivity: human, rat, monkey, mouse
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells; western blot: 0.5-1.0 μg/mL using whole extracts of human HepG2 and rat NRK cells
• UniProt accession no.: P23284
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PPIB(5479) ; mouse ... Ppib(19035)

Description

General description

Anti-Cyclophilin B is produced in rabbit using as the immunogen a synthetic peptide corresponding to a fragment of human Cyclophilin B conjugated to KLH. Cyclophilin B (CypB) is a 21 kDa protein encoded by the PPIB gene, that belongs to the cyclophilin family, a highly conserved class of proteins originally identified as cellular binding proteins for the immunosuppressive drug cyclosporin A. CypB is mainly located within the endoplasmic reticulum (ER) of all cell types. CypB is involved in inflammation, viral infection and cancer.

Application

Anti-Cyclophilin B antibody produced in rabbit has been used:

• immunoblotting
• immunofluorescence
• in immunocapture-polymerase chain reaction (PCR) to study the interactions between CypB and Tomato yellow leaf curl virus (TYLCV) coat protein (CP).

Biochem/physiol Actions

Cyclophilins are peptidyl-prolyl cistrans isomerases (PPIases) that assist protein folding by catalysing the isomerization of peptidyl-proline bonds. Cyclophilin B (CypB) also plays an important role in protecting cells against ER stress via its PPIase activity. Mutations in the PPIB gene give rise to recessive forms of osteogenesis imperfecta and knockdown of this gene decreases cell growth, proliferation and migration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable