SAB4200097

Anti-NOX1 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(s): Anti-GP91-2, Anti-MOX1 (mitogenic oxidase 1), Anti-NADPH oxidase 1, Anti-NOH1 (NADPH oxidase homolog 1)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~72 kDa
• species reactivity: human
• concentration: ~1.5 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon; western blot: 0.5-1.0 μg/mL using HS68 cell extracts
• UniProt accession no.: Q9Y5S8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NOX1(27035) ; mouse ... Nox1(237038) ; rat ... Nox1(114243)

Description

General description

NADPH oxidase 1 (NOX1) is encoded by the gene mapped to human chromosome Xq22.1. The protein belongs to the family of NADPH oxidases and is co-localized with caveolin in punctate patches on the surface and laterally on the cellular margins. NOX1 is mainly expressed in colon epithelium.

Application

Anti-NOX1 antibody produced in rabbit has been used in:

• Immunocytochemistry.
• Immunoprecipitation[1]
• Immunoblotting.[1]

Anti-NOX1 antibody produced in rabbit may be used in immunofluorescence and immunohistochemistry.

Biochem/physiol Actions

NADPH oxidase 1 (NOX1) requires two cytosolic regulators NADPH oxidase activator 1(NOXA1) and NADPH oxidase organizer 1 (NOXO1) as well as Ras-related C3 botulinum toxin substrate 1 (Rac1) for its activity. NOX1 and NOX2 promote neurotoxic activation of microglia suggesting that they play a central role during neuroinflammatory states and in amyotrophic lateral sclerosis (ALS). In ALS mice deletion of either NOX1 and NOX2 gene have been shown to significantly slowed disease progression and improved survival.

NOX1 catalyzes the production of hydrogen peroxide (H2O2). Overexpression of the protein leads to the production of both superoxide and H₂O₂ and triggers angiogenic switch, mediating vascularization and rapid expansion of the tumor. Elevated expression of NOX1 has been observed in the brain of Parkinson′s disease (PD) patients.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable