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S7899

Sodium acetate buffer solution

pH 5.2±0.1 (25 °C), Molecular Biology, 3 M, 0.2 μm filtered

Synonym(s):

Sodium acetate buffer 3M

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About This Item

CAS Number:
NACRES:
NA.52
PubChem Substance ID:
eCl@ss:
32129211
UNSPSC Code:
12161700
MDL number:
Beilstein/REAXYS Number:
3786729
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grade

Molecular Biology

Quality Segment

sterility

0.2 μm filtered

form

liquid

concentration

3 M

pH

5.2±0.1 (25 °C)

suitability

suitable for DNA precipitation

storage temp.

room temp

SMILES string

[Na+].CC(O)=O.CC([O-])=O

InChI

1S/2C2H4O2.Na/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+1/p-1

InChI key

BHZOKUMUHVTPBX-UHFFFAOYSA-M

General description

Sigma′s Sodium Acetate Buffer is a 3M solution prepared with 18 megaohm water and filter-sterilized.

Application

Sodium acetate buffer solution is suitable for precipitation of DNA, elution of proteins and for use in molecular biology applications such as ChIP-on-ChIP assay[1] and in vitro enzyme inhibition assay.[2][3]

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This Item
567422S240471196
grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

concentration

3 M

concentration

-

concentration

3 M

concentration

~3 M in H2O

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

room temp

storage temp.

10-30°C

storage temp.

2-8°C

storage temp.

-

pH

5.2±0.1 (25 °C)

pH

-

pH

7.0±0.05 (25 °C)

pH

7.0-8.0 (25 °C, 3 M in H2O)


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pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certificates of Analysis (COA)

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Questions

1–3 of 3 Questions  
  1. what is the proper way to store the Sodium acetate buffer solution and how long can I keep it without losing its properties after opening;

    1 answer
    1. The product is chemically stable under standard ambient conditions (room temperature). Section 7 of the Safety Data Sheet provides information on handling and storage. The product is covered under a 1-year warranty if stored correctly. https://www.sigmaaldrich.com/sds/sigma/s7899?userType=anonymous

      Helpful?

  2. What is the concentration of the acetic acid in this solution?

    1 answer
    1. This product is prepared from a powdered sodium acetate compound. The material is CAS 127-09-3 with the molecular formula of C2H3NaO2. No additional acetic acid is used in the formulation of this buffer.

      Helpful?

  3. 여기 용액안에 Triton-X100가 들어가 있나요?

    1 answer
    1. As mentioned on the Product Datasheet located in the 'DOCUMENTATION' section, this product is a 3 M solution prepared with sodium acetate (Molecular Biology grade) and 18 megaohm water. Triton-X 100 has not been added to this formulation.

      Helpful?

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