Suitable replacements for the deleted Blueing Reagent (65354-85) are S5134 and 106329. Scott's Tap Water Concentrate is diluted 1 to 10 before use. 106329 is typically dissolved in water at a concentration of 15 grams per liter. It is also possible to use a 0.2% solution of ammonium hydroxide or a saturated solution of lithium carbonate.
S5134
Scott′s Tap Water Substitute Concentrate
10 ×
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About This Item
NACRES:
NA.47
UNSPSC Code:
41116124
Pricing and availability is not currently available.
form
powder
Quality Segment
IVD
for in vitro diagnostic use
concentration
10 ×
technique(s)
titration: suitable
pH
7.0-7.8
application(s)
hematology
histology
storage temp.
room temp
Application
Used as a "blueing reagent" in hematoxylin and eosin staining procedures. Scott′s Tap Water Substitute Concentrate has been used in transferase−mediated dUTP nick end labeling (TUNEL) assay. It has been used in immunohistochemical analysis.
Preparation Note
Scott′s Tap Water Substitute is prepared by mixing 1 part of Scott′s Tap Water Substitute Concentrate with 9 parts deionized water.
Other Notes
MgSO4 • 7H2O, 200 g/L + NaHCO3, 20 g/L
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) microbe id | staining: suitable | technique(s) - |
| concentration 10 × | concentration 10 % (w/v) | concentration - | concentration 1% in ethanol |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) hematology |
| form powder | form solution | form solution | form solution |
| IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD for in vitro diagnostic use |
| Quality Level 500 | Quality Level 500 | Quality Level 200, 500 | Quality Level 200, 500 |
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Product 65354-85 has been deleted. What is a suitable replacement product?
1 answer-
Helpful?
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Is there a direct replacement for S5134 Scott's Tap Water Substitute?
1 answer-
There is no direct replacement for the product in question. However, a solution with 15 grams of sodium bicarbonate per liter of water serves exactly the
Helpful?
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Must Scott's Tap Water Substitute be diluted with deionized water, and must the solution be prepared fresh for each day?
1 answer-
The Scott's Tap Water Substitute Concentrate should be diluted with deionized water. When using distilled water, it is advisable to check the pH after preparing the 1X solution. Alternatively, the solution prepared with distilled water can be used to blue tissue sections after staining with hematoxylin. If the nuclei do not turn blue, it may indicate that the pH of the solution is not alkaline enough to convert the nuclei color to blue.
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