S0439

SUMO-1 human

≥95% (SDS-PAGE), recombinant, expressed in E. coli (GST-tagged)

• Synonym(s): Sentrin-1
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD03458656

Properties

• recombinant: expressed in E. coli (GST-tagged)
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• mol wt: 38.5 kDa
• UniProt accession no.: P63165
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... SUMO1(7341)

Description

General description

Small ubiquitin-like modifier 1 (SUMO-1), a protein, is one of the 5 SUMO paralogs expressed in mammalian cells with a low but significant homology with ubiquitin. This SUMO-1-GST fusion protein is produced from a DNA sequence corresponding to human SUMO-1 fused to a glutathione S-transferase (GST)-tag and is expressed in E. coli cells.

Application

SUMO-1 human has been used in pre-absorption for negative immunostaining to confirm the specificity of the small ubiquitin like modifier 1 (SUMO-1) antibody.

Biochem/physiol Actions

Conjugated to a variety of proteins by UbcH9, including RanGAP1, IκBα, and PML without the requirement of an equivalent E3 ubiquitin ligase.

Small ubiquitin-like modifier 1 (SUMO-1) plays a key role in modulating survival, migration, and signal transduction in some cell lines. It can prevent in vitro migration and invasion of RA fibroblast-like synoviocytes (FLSs). Higher expression of SUMO-1 may result in joint destruction in rheumatoid arthritis (RA).

Physical form

Solution in 50 mM HEPES, pH 8.0, 150 mM NaCl and 1 mM DTT.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)