Minor adjustments were introduced to the procedure, specifically regarding the volumes of reagents used in certain steps. Nevertheless, the methodology remains essentially unchanged compared to the previous version. These modifications are of little significance and do not affect the duration or efficiency of the method, both of which remain equivalent to those previously established.
PLN70
GenElute™ Plasmid Miniprep Kit
sufficient for 70 purifications
Sustainability features
Synonym(s):
GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
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About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
Pricing and availability is not currently available.
usage
sufficient for 70 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Sustainability features
technique(s)
DNA purification: suitable
test parameters
: <30 min kit run time, sample volume: 1-5 mL
storage temp.
15-25°C
General description
The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.
Features and Benefits
- Purified plasmid DNA in less than 30 minutes
- Purify up to 15 μg of plasmid DNA
- No detectable genomic DNA or RNA contamination
- No phenol/chloroform extraction or alcohol precipitation required
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| description sufficient for 70 purifications | description sufficient for 350 purifications | description sufficient for 350 preparations | description sufficient for 70 preparations |
| usage sufficient for 70 purifications | usage sufficient for 350 purifications | usage sufficient for 350 preparations | usage sufficient for 70 preparations |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable | technique(s) DNA extraction: suitable |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| test parameters : <30 min kit run time, sample volume: 1-5 mL | test parameters : <30 min kit run time, sample volume: 1-5 mL bacterial culture | test parameters : <30 min kit run time, sample volume: 1-5 mL bacterial culture | test parameters - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class
3 - Flammable liquids
WGK
WGK 2
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