P4380

Protamine sulfate

powder

• Synonym(s): Salmine, Salmine from salmon
• CAS Number: 53597-25-4
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00132091

Properties

• Product Name: Protamine sulfate salt from salmon, Grade II
• biological source: fish (salmon)
• Quality Segment: 200
• type: Grade II
• form: powder
• color: white to off-white
• solubility: 6 M HCl: 25 mg/mL, clear, colorless to light yellow

Description

General description

Protamine sulfate (PS), a polycationic, positively charged protein is mostly made up of poly(l-arginine). It is obtained from salmon sperm protein.

Application

Protamine sulfate salt from salmon has been used in the generation of protamine silica nanocomposites.

May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1.

Biochem/physiol Actions

Protamine sulfate is highly preferred in cardiovascular surgical procedures to reverse the anticoagulant properties of heparin. It is used for biomedical and biotechnological applications due to its biodegradablity.

Protamine sulfate is a small cationic protein that binds and precipitates DNA. Neutralizes the anticoagulant activity of heparin. Inhibits lipoprotein lipase.

Analysis Note

May contain insolubles as well as histones.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)