P2893

JumpStart^™ Taq ReadyMix^™

Complete optimized reagent for hot-start PCR at 2X concentration

• Synonym(s): hot start PCR master mix, hot start master mix, hot start taq master mix, mutiplex PCR
• MDL number: MFCD00166026
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• form: liquid
• usage: sufficient for 100 reactions; sufficient for 400 reactions
• feature: Multiplex PCR; dNTPs included; hotstart
• concentration: 2.5 units/reaction (50 μL reaction volume)
• technique(s): PCR: suitable
• color: colorless
• suitability: suitable for (quantitative PCR)
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

JumpStart^™ Taq ReadyMix^™ is a ready-to-use 2X master mix that contains JumpStart^™ Taq DNA polymerase, 99% pure dNTPs, reaction buffer and JumpStart^™ Taq antibody. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

Application

JumpStart^™ Taq ReadyMix^™ has been used in quantitative polymerase chain reaction (PCR). It has also been used to identify fibroblast cell surface adenosine receptor (AR) types under hypoxia. It is also suitable:

• For PCR amplifications that require reduced non-specific amplification
• For multiplex PCR
• For reduction of primer dimers

Features and Benefits

• The master mix allows consistency from one reaction to the next
• Reduced preparation time and reduced risk of contamination from multiple pipetting steps
• Designed to minimize non-specific amplification and contamination
• Increased specificity and target yield
• Reduced primer dimers
• Reduced set-up time as compared to manual or wax Hot Start methods
• Allows for room temperature set-up
• Ideal for high throughput, quantitative PCR applications

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL

Other Notes

JumpStart Taq ReadyMix is a prepared solution combining the performance benefits of hot start PCR with the convenience of a ReadyMix. The mix includes JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. JumpStart Taq Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq polymerase does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The hot start mechanism allows for room temperature set up, making it ideal for high throughput applications. To prepare a 50 μL reaction, simply add 25 μL of ReadyMix to template, primers and water for a final reaction volume of 50 μL.

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To prepare a 50 μL reaction, simply add 25 μL of ReadyMix^™ to template, primers and water for a final reaction volume of 50 μL.
This product has been validated in quantitative PCR, but may require supplementation with magnesium chloride solution, 25 mM, Catalog Number M8787, a suitable fluorescent probe, and, if desired, an internal reference dye, Catalog Number R4526. View more detailed information on JumpStart Taq ReadyMix products at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable