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Neuraminidase Agarose from Clostridium perfringens (C. welchii)

Name: Neuraminidase Agarose from <I>Clostridium perfringens (C. welchii)</I>
Brand: SIGMA

Type VI-A, ammonium sulfate suspension

Synonym(s):

Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.18 (BRENDA, IUBMB)

MDL number:

MFCD00131711

Specific activity:

20-60 units/g agarose

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Properties

type

Type VI-A

form

ammonium sulfate suspension

specific activity

20-60 units/g agarose

extent of labeling

0.6-1.8 units per mL gel, 20-60 units per g agarose

matrix

beaded agarose

storage temp.

2-8°C

Description

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Agarose-linked neuraminidase from Clostridium perfringens can be used for the detection of total and desialylated sex hormone-binding globulin in human serum samples. [1]

Neuraminidase from Clostridium perfringens has been used in a study to assess a genetic polymorphism of human serum glycoprotein (inter-α-trypsin-inhibitor). [2] It has also been used in a study to investigate the inhibition of neuraminidase by chemically sulphated glycopeptides. [3]

Physical form

Suspension in 2.0 M (NH₄)₂SO₄ solution, pH 7.0.

Preparation Note

Prepared from Neuraminidase, Type VI (N 3001).

Other Notes

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

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This Item	N2133	N5631	N3001
Sigma-Aldrich N5254 Neuraminidase Agarose from Clostridium perfringens (C. welchii)	Sigma-Aldrich N2133 Neuraminidase from Clostridium perfringens (C. welchii)	Sigma-Aldrich N5631 Neuraminidase from Clostridium perfringens (C. welchii)	Sigma-Aldrich N3001 Neuraminidase from Clostridium perfringens (C. welchii)
specific activity 20-60 units/g agarose	specific activity ≥50 units/mg protein (using 4MU-NANA)	specific activity ≥10 units/mg protein (using 4MU-NANA), ≥3.5 units/mg protein (mucin)	specific activity ≥2 units/mg protein (mucin), ≥6 units/mg protein (using 4MU-NANA)
form ammonium sulfate suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
extent of labeling 0.6-1.8 units per mL gel, 20-60 units per g agarose	extent of labeling -	extent of labeling -	extent of labeling -
type Type VI-A	type Type X	type Type VIII	type Type VI

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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