The protocol indicates that 5 uL of the probe should be added to the working reagent. This is described in Table 2, located on page 3 of the datasheet. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/429/213/mak443pis-ms.pdf
MAK443
L-Lactate Assay Kit
Sufficient for 100 fluorometric tests
Synonym(s):
ʟ-Lactic Acid Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
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Quality Level
input
cell culture medium
plasma
serum
urine
detection method
fluorometric
relevant disease(s)
diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer
storage temp.
−20°C
General description
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.
Application
The L-Lactate Assay Kit may be used for:
- Lactate Metabolism Research
- Tissue Metabolism Research
- Drug Discovery
Features and Benefits
- Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
- Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
- High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.
Suitability
The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.
Principle
The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
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The kit contains a probe, but no instructions in the protocol of when/how much to add. Please advise on the probe-adding step?
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