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4-Methylumbelliferyl phosphate

Name: 4-Methylumbelliferyl phosphate
Brand: SIGMA

phosphatase substrate, fluorogenic, ≥98% (HPLC), powder

Synonym(s):

4-Methylumbelliferyl-phosphoric acid

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₉O₆P

CAS Number:

3368-04-5

Molecular Weight:

256.15

NACRES:

NA.32

PubChem Substance ID:

24897292

UNSPSC Code:

12352204

EC Number:

222-137-7

MDL number:

MFCD00016969

Beilstein/REAXYS Number:

1687229

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Properties

Product Name

4-Methylumbelliferyl phosphate, phosphatase substrate

Quality Segment

200

assay

≥98% (HPLC)

form

powder

solubility

water: 25 mg/mL, clear to very slightly hazy, colorless

fluorescence

λ_ex 319 nm; λ_em 384 nm (pH 9.1), λ_ex 360 nm; λ_em 449 nm (Reaction product)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12

InChI

1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)

InChI key

BCHIXGBGRHLSBE-UHFFFAOYSA-N

Description

General description

Fluorogenic substrate for phosphatases.

Application

4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay. It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses.

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This Item	M3168	M8168	M7133
Sigma-Aldrich M8883 4-Methylumbelliferyl phosphate	Sigma-Aldrich M3168 4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System	Sigma-Aldrich M8168 4-Methylumbelliferyl phosphate disodium salt	Sigma-Aldrich M7133 4-Methylumbelliferyl sulfate potassium salt
description phosphatase substrate	description liquid	description phosphatase substrate	description sulfatase substrate
assay ≥98% (HPLC)	assay -	assay ≥98% (TLC)	assay ≥99% (HPLC)
solubility water: 25 mg/mL, clear to very slightly hazy, colorless	solubility -	solubility water: 50 mg/mL, clear, colorless	solubility water: 5 mg/mL, clear, colorless
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
form powder	form liquid	form powder	form powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
fluorescence λ_ex 319 nm; λ_em 384 nm (pH 9.1), λ_ex 360 nm; λ_em 449 nm (Reaction product)	fluorescence -	fluorescence λ_ex 319 nm; λ_em 384 nm (pH 9.0), λ_ex 360 nm; λ_em 449 nm (Reaction product)	fluorescence λ_ex 334 nm; λ_em 370 nm (pH 10.4), λ_ex 360 nm; λ_em 449 nm (Reaction products)

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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