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M5815

Sigma-Aldrich

Monoclonal Anti-Myogenin antibody produced in mouse

clone F12B, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
MFCD01322371
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

F12B, monoclonal

form

buffered aqueous solution

mol wt

antigen 34 kDa

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections
immunoprecipitation (IP): 2 μg using 1 mg protein lysate
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1 μg/mL

isotype

IgG1

UniProt accession no.

P15173

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYOG(4656)
mouse ... Myog(17928)
rat ... Myog(29148)

General description

Myogenin belongs to the basic helix-loop-helix (bHLH) superfamily.
Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons.

Immunogen

rat recombinant myogenin (amino acids 30-224).

Application

Monoclonal Anti-Myogenin antibody produced in mouse has been used in immunohistochemistry, immunofluorescence and western blotting.

Biochem/physiol Actions

Myogenin acts as a biomarker in rhabdomyosarcoma (RMS). It has a role in developmental processes, such as hematopoiesis, neurogenesis, myogenesis, tumor cell invasion and metastasis.
Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pricing

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Shen et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 49(2), e5124-e5124 (2016-02-13)
This study evaluated the effect of muscle satellite cells (MSCs) overexpressing myogenin (MyoG) on denervated muscle atrophy. Rat MSCs were isolated and transfected with the MyoG-EGFP plasmid vector GV143. MyoG-transfected MSCs (MTMs) were transplanted into rat gastrocnemius muscles at 1
Implantation of muscle satellite cells overexpressing myogenin improves denervated muscle atrophy in rats
Shen H, et al.
Brazilian journal of medical and biological research, 49(2) (2016)
Calcium in the Regulation of Gene Expression
Encyclopedia of Toxicology, 126(9), 310-315 (2013)
W Glenn McCluggage et al.
Histopathology, 63(4), 545-550 (2013-08-16)
Myogenin (myf4) is a nuclear transcription factor that is considered to be a sensitive and highly specific marker for skeletal muscle differentiation. Following the identification of focal strong nuclear staining with myogenin in two fibroepithelial polyps of the lower female
Adriana Pertille et al.
Revista brasileira de fisioterapia (Sao Carlos (Sao Paulo, Brazil)), 16(6), 495-501 (2012-10-13)
The aging process and its associated morphophysiological changes trigger a reduction in the regenerative ability of the satellite cells, a reduction of vascular tissue and an increase in the production of fibroblasts, developing a cellular environment unfavorable for muscle regeneration.
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