This product undergoes a filtration step using a 0.2 µm filtration membrane. However, sterility testing is not performed on the packaged material.
M5353
Mitomycin C
liquid, non-animal origin, suitable for cell culture, BioReagent
Synonym(s):
Ametycine, MMC, MitoExtra, Mitocin C, Mitomycin, Mitonco, Mitoplus, Mitozytrex, Mutamycin
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.76
Pricing and availability is not currently available.
Product Name
Mitomycin C ready made solution, 10 mg/mL in DMSO, ≥98% (HPLC), Cell culture tested
Quality Segment
assay
≥98% (HPLC)
form
liquid
storage condition
protect from light
concentration
10 mg/mL in DMSO
storage temp.
−20°C
General description
Mitomycin C (MMC) produced by Streptomyces caespitosus is an anticancer antibiotic agent which inhibits proliferation of various cell types in vitro. MMC is DNA alkylating agent, that causes cross linking of complementary DNA strands resulting in: mutagenesis, inhibition of DNA synthesis, initiation of DNA repair events, and activation of apoptosis. Furthermore, MMC has an effect on ribosomal RNA leading to protein synthesis inhibition.
MMC is capable of inhibiting cell proliferation of human or murine embryonic fibroblast cells at concentration range of 0.2-20μg/mL. These treated cells are subsequently used as an adherent feeder layer in long-term culture assays mainly for growth of pluripotent stem cells. Feeder layer cells support the target cells by releasing growth factors to the cell culture and detoxification of the medium. Moreover, feeder layer cells play a role in ECM proteins synthesis that controls cell culture growth. In addition, feeder layer cells are used as a substrate for attachment of the target cells.
MMC also was used for BLC (basal-like cancer) cell line.
MMC as antibiotic agent showed a potent activity against MDR gram-negative bacteria like P. aeruginosa (2 μg/mL), E.Coli (0.5μg/mL) and A. baumannii (16μg/mL). MMC also has a strong activity against gram positive bacteria (0.2μg/mL).
Technical information
1.Mitomycin C ready made solution provides a safer alternative to the powder form by preventing hazardous particles exposure.
2.Please avoid repeated freeze thaw cycles of this product.
3.Mitomycin C solutions are light sensitive and should kept in the dark.
4.It is recommended to keep Mitomycin C solutions at pH 6-7.
5.The recommended concentration of Mitomycin C in cell culture assays is 0.2-20μg/mL. (Ref.8,9,12,13) Therefore, it is recommended to dilute the ready made solution 1:500-50,000.
MMC is capable of inhibiting cell proliferation of human or murine embryonic fibroblast cells at concentration range of 0.2-20μg/mL. These treated cells are subsequently used as an adherent feeder layer in long-term culture assays mainly for growth of pluripotent stem cells. Feeder layer cells support the target cells by releasing growth factors to the cell culture and detoxification of the medium. Moreover, feeder layer cells play a role in ECM proteins synthesis that controls cell culture growth. In addition, feeder layer cells are used as a substrate for attachment of the target cells.
MMC also was used for BLC (basal-like cancer) cell line.
MMC as antibiotic agent showed a potent activity against MDR gram-negative bacteria like P. aeruginosa (2 μg/mL), E.Coli (0.5μg/mL) and A. baumannii (16μg/mL). MMC also has a strong activity against gram positive bacteria (0.2μg/mL).
Technical information
1.Mitomycin C ready made solution provides a safer alternative to the powder form by preventing hazardous particles exposure.
2.Please avoid repeated freeze thaw cycles of this product.
3.Mitomycin C solutions are light sensitive and should kept in the dark.
4.It is recommended to keep Mitomycin C solutions at pH 6-7.
5.The recommended concentration of Mitomycin C in cell culture assays is 0.2-20μg/mL. (Ref.8,9,12,13) Therefore, it is recommended to dilute the ready made solution 1:500-50,000.
Physical form
Liquid - In DMSO
Disclaimer
Light sensitive
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This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form powder | form liquid |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay - | assay - |
| concentration 10 mg/mL in DMSO | concentration 10 mg/mL in H2O | concentration - | concentration 10 mg/mL in H2O |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| storage condition protect from light | storage condition - | storage condition - | storage condition protect from light |
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Is the Mitomycin C (#M5353-0.2ML) ready to use sterile? Is it necessary to filter the media or solution where it is added?
1 answer-
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is the concentration 10 miligram per mililiter or 10 mikrogram per mililiter?
1 answer-
The described concentration of 10mg/mL is equivalent to 10 milligrams per milliliter.
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what should Mitomycin C(M5353) ready made solution be dilulted with? How long can it be stored ? Can we thaw and reuse it again? What temeprature should the diluted silution be stored at ?
1 answer-
This product may be further diluted in culture medium or buffer at pH 6 - 9. Upon dilution, the solution is stable for up to 1 week when stored refrigerated and protected from light. At the original 10 mg/mL concentration, the product may be stored at −20°C in aliquots at least until the recommended retest date is published in the Certificate of Analysis. Avoid repeated freeze/thaw cycles. Please see the link below to review additional information regarding handling and stability of Mitomycin C:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/266/701/m4287pis.pdfHelpful?
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Can I keep the solution dissolved in DMSO for longer if I keep it frozen?
1 answer-
Please see the 'Properties' section of the Product Detail Page. Product M5353 is provided at 10mg/mL in DMSO. It needs to be kept frozen at -20°C to remain stable until the printed Retest date provided on the Certificate of Analysis.
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