M2444

Anti-MDC1 antibody, Mouse monoclonal

clone MDC1-50, purified from hybridoma cell culture

• Synonym(s): Anti-Mediator of DNA Damage Checkpoint Protein 1
• MDL number: MFCD07370941
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: MDC1-50, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~250 kDa (2-3 bands)
• species reactivity: monkey, human
• packaging: antibody small pack of 25 μL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of G361 cells
• isotype: IgG2a
• UniProt accession no.: Q14676
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MDC1(9656)

Description

General description

Monoclonal Anti-MDC1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant human MDC1 fragment. Mediator of DNA damage checkpoint protein 1 (MDC1) contains 2089 amino-acid residues with a predicted molecular weight of 226.4 kDa. The protein contains an FHA (forkhead-associated) domain at its amino terminus and two BRCT (BRCA1 carboxyl terminal) domains at its carboxy terminus.

Specificity

Mouse monoclonal clone MDC1-50 anti-MDC1 antibody recognizes human and monkey MDC1.

Immunogen

recombinant human MDC1 fragement (amino acids 2-200).

Application

Monoclonal Anti-MDC1 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA
• immunofluorescence
• western blotting
• immunoprecipitation
• immunocytochemistry

Mouse monoclonal clone MDC1-50 anti-MDC1 antibody is used to tag mediator of DNA damage checkpoint protein 1 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques such as ELISA, immunoblotting (approx. 250 kDa, 2-3 bands), immunoprecipitation and immunocytochemistry. It is used as a probe to determine the roles of mediator of DNA damage checkpoint protein 1 in DNA repair and genomic stability.

Biochem/physiol Actions

Genomic instability caused by the disruption of mechanisms that regulate cell-cycle checkpoints, DNA repair and apoptosis may lead to the development of cancer. ATM and ATR protein kinases are mediated to DNA damage sites by molecular adapters or mediators proteins such as Histone H2AX, Claspin and BRCTmotif containing molecules such as 53BP1, BRCA1, and MDC1 (mediator of DNA damage checkpoint protein 1). MDC1 interacts with the MRE11 complex (containing MRE11, RAD50 and NBS1 proteins). The MRE11 complex is involved in the detection repair and signaling of DNA damage. Upon ionizing radiation MDC1 is hyperphosphorylated by ATM and localizes to nuclear foci together with the MRE11 complex, phosphorylated H2AX and 53BP1. A radio resistant DNA synthesis (RDS) phenotype in cells is formed by down regulation of MDC1 protein expression by siRNA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable