The product can be used at a maximum pressure of 15 psi without crushing the beads, although compression may occur at this pressure.
L7634
α-Lactose-Agarose
saline suspension
Synonym(s):
alpha-lactose resin
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About This Item
Pricing and availability is not currently available.
biological source
bovine milk
form
saline suspension
technique(s)
affinity chromatography: suitable
matrix
6% beaded agarose
matrix activation
divinyl sulfone
matrix attachment
hydroxyl
matrix spacer
5 atoms
capacity
6-25 mg/mL binding capacity (lectin from Arachis hypogaea)
suitability
suitable for chromatography
storage temp.
2-8°C
Application
α-Lactose-agarose has been used:
- in protein chromatography to purify galectins from lysed bacteria cells
- to capture ricin based on lectin-carbohydrate affinity
- to study microneme proteins and host-parasite cell surface receptors
α-lLactose-agarose is used in protein chromatography, affinity chromatography and carbohydrate matrices. α--Lactose-agarose has been used to study microneme proteins and host-parasite cell surface receptors.
Physical form
Suspension in 0.15 M NaCl, 0.01 M sodium phosphate, pH 6.8, containing 0.02% sodium azide
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This Item | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) - |
| biological source bovine milk | biological source birch/beech wood hydrolysate | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| suitability suitable for chromatography | suitability suitable for chromatography | suitability - | suitability - |
| form saline suspension | form saline suspension | form saline suspension | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the maximum flow rate for α-Lactose-Agarose (#L7634)?
1 answer-
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Can this resin be cleaned and re-used?
1 answer-
This product is assayed for the ability to bind lectin from Arachis hypogaea. The lectin is loaded in a 0.5 M sodium chloride, 0.01 M sodium phosphate buffer, pH 6.8. The column is washed with the same buffer. The lectin is eluted in the same buffer containing 0.1 M alpha-lactose.
The resin can be regenerated by washing with 3-5 column volumes of 50 mM acetic acid, pH 3.0 containing 0.5 M sodium chloride, followed by 50 mM borate buffer at pH 7.0. The resin can also be washed with 6 M guanidine hydrochloride solution, adjust to pH 3 with acetic acid. The ligand on the resin is susceptible to hydrolysis at pH above 10.
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